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Lhca3 antibody

Lhca3 Reactivity: Arabidopsis thaliana, Barley, Green bean, Oryza sativa, Pisum sativum, Spinach, Zea mays WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN93513
  • Target
    Lhca3 (psi Type III Chlorophyll A/B-Binding Protein (Lhca3))
    Reactivity
    Arabidopsis thaliana, Barley, Green bean, Oryza sativa, Pisum sativum, Spinach, Zea mays
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
    Purification
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
    Isotype
    IgG
  • Application Notes
    Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
  • Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

    Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

  • Target
    Lhca3 (psi Type III Chlorophyll A/B-Binding Protein (Lhca3))
    Synonyms
    LHCA3*1 antibody, LHCI-680 antibody, T25B24.12 antibody, T25B24_12 antibody, photosystem I light harvesting complex gene 3 antibody, PSI type III chlorophyll a/b-binding protein antibody, LHCA3 antibody
    Background
    AGI Code: At1g61520
    The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
    Molecular Weight
    expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
    UniProt
    Q9SY97
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