Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity
Chlamydomonas reinhardtii
Characteristics
Expected / apparent Molecular Weight of the Antigene: 32.7 kDa/24.1 kDa
Purification
affinity purified
Immunogen
KLH-comjugated synthetic peptide chosen from a sequence of Chlamydomonas reinhardtii Lci5 protein A8IGD9
Isotype
IgY
Application Notes
1: 2000 - 1: 5000 with standard ECL (WB)
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS pH 8.5 + 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
Storage
4 °C
Storage Comment
store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Target
LCI5
(Low-CO2-Inducible Protein (LCI5))
Alternative Name
Lci5
Synonyms
low-CO2-inducible protein antibody, LCI5 antibody
Background
Lci5 protein is encoded by the nuclear gene lci5, which is induced during the acclimation to low CO2 conditions in the green alga Chlamydomonas reinhardtii. The Lci5 protein is peripherally associated with the stroma side of thylakoid membranes. In addition, this protein is heavily phosphorylated (7 sites, 3 Threonine and 4 serine residues, as shown by mass spec anlaysis) in cells growing under low CO2 concentrations in the medium or during state transitions (state 2). Microarray analysis showed that the gene is up-regulated 3 times during the acclimation to low CO2 conditions.