ELISA Kits

Compare and order fully validated and ready-to-use ELISA Kits (enzyme-linked immunosorbent assay kits). On antibodies-online you will find a wide range of reliable kits to quickly and easily detect or quantify proteins in biological samples or solutions. New to ELISAs? Visit our ELISA beginners guide!

ELISA Kits by Species Reactivity

antibodies-online.com supplies Enzyme Linked Immunosorbent Assay (ELISA) kits for the detection of numerous targets such as antigens, proteins and peptides. We offer a wide selection of species reactivity. Click on a species to go to the corresponding products. Then you can easily filter by target and other properties.

• Camel

  Cat

  Chemical

  Chicken

  Cow

  Dog
  Duck

  Fish

  Goat

  Guinea pig

  Hamster

  Hormone
• Horse

  Human

  Mammalian

  Monkey

  Mouse

  Pig
  Plant

  Rabbit

  Rat

  Sheep

  Zebrafish

We offer ELISA kits for a large number of other, less frequently used species. Use our search to find the right product for you.

ELISA Kits by Sample Type

Click on a sample type to go to the corresponding products. Then you can easily filter by target and other properties.

• Cell Culture Cells

  Cell Culture Lysate

  Cell Culture Supernatant

  Cell Lysate
  Cell Samples

  Cerebrospinal Fluid

  Milk

  Plasma
• Saliva

  Serum

  Suspension Cell Culture

  Tissue Homogenate
  Tissue Lysate

  Tissue Samples

  Urine

We support you with ELISA Kits for many other sample types such as food, sweat, blood, tears, or plant tissue. Use our search to find the right product for you.

Relevant Categories and Topics

To select the appropriate ELISA Kit, you can use our convenient search function. Here you can filter for example by species / reactivity, ELISA Kit type, sample type and other criteria. Below you will find some relevant topic pages and pathways.

• Apoptosis Pathway

  Inflammasome

  Cell Division Cycle

  Complement System
  DNA Damage Repair

  MAPK Signaling

  Infectious Disease

  Neurodegenerative Disease
• Protein Tumor Markers

  TLR Signaling

  WNT Signaling

  Hedgehog Signaling
  JAK-STAT Signaling

  BCR Signaling

  Cancer Immune Checkpoints

Featured ELISA Kits with comprehensive Validation Data

Here you will find a selection of the most popular products, such as anti LPS ELISA kits, anti IL1b ELISA kits, anti IL8 ELISA kits and many more.

antibodies-online supplies you with ELISA Kits for more than 3,000 different targets. You can search for the ELISA Kit you need by target name, species, detection method, sample types, validation data, and other features. In case you need assistance, our customer service team of PhD biologists is looking forward to your request via phone or email to support you in your research work.

ELISA Kit: Component Validation

When it comes to ELISA Kits, reliability is one of the most important aspects. antibodies-online offers you a portfolio of selected ELISA Kits with validated components and the corresponding validation data. So you can be sure that your kit will work as expected. Please see below the validation data of the components of our TNF ELISA Kit (ABIN6574141) as an example.

Mouse Capture Antibody from the kit in WB with Positive Control: Eukaryotic TNFa protein.

Mouse Detection Antibody from the kit in WB with Positive Control: Sample Eukaryotic TNFa protein.

SDS-PAGE of Protein Standard from the kit (Highly purified E. coli-expressed recombinant rat TNFa).

WB of Protein Standard: different control antibodies against Highly purified E. coli-expressed recombinant rat TNFa.

antibodies-online.com - your supplier for high quality ELISA and assay kits. With more than 15 years of experience we are the competent partner for your research project. Whatever you are looking for, you will find the right product with us. We guarantee the full function of our ELISA Kits and therefore offer you a full money-back guarantee. If you have any questions about our products or the application in your specific project, our competent PhD customer service is at your disposal. Please contact us via email or phone.

When to build an assay, when to buy an ELISA Kit?

Once you’ve decided that you’d like to use an ELISA, you need to decide whether you’ll use a commercially available ELISA Kit, or try to compile an assay on your own from scratch. There are a few reasons that one might want to try to generate an assay de novo rather than purchasing a pre-made solution. If you need to fine tune your assay outside the specifications in a commercially available kit, if you need tight control over the specific nature of the components that go in to your assay, or if you need to use a specific or custom antibody in your assay you might want to consider investing the time and effort necessary to generate an assay de novo.

However, if you’re not limited to the criteria above, you might want to consider purchasing a commercially available assay for simplicity’s sake. You should consider purchasing a pre-made kit if you want to:

• Save time: Optimization of custom ELISA is very time consuming. Even for an experienced scientist with compatible reagents, a new assay will generally require hours or days of optimization before it is ready for use. That’s all time that you could be using to collect, analyze, interpret, and publish your data. Carefully consider the value of your time, and the time of the technicians and students who will be building and performing the assay.
• Ensure reagent compatibility: A pre-made ELISA Kit is guaranteed to contain reagents that are compatible with each other. When compiling your own assay, even if you choose high quality reagents you may accidentally stumble upon incompatible products (e.g. two antibodies that recognize a similar epitope and block each other due to steric hindrance).
• Avoid frustrating quality-control issues: When generating an assay from scratch, you'll likely be sourcing different reagents from several different places. And after spending all the time necessary to track down each of the various reagents you need, even a single bad buffer could ruin your experiment, requiring countless hours of troubleshooting to trace the source of the problem. By contrast, every pre-made ELISA Kit from antibodies-online is covered by our money back guarantee. In the unlikely event that you do experience a problem, our technical support staff will handle the troubleshooting.
• Save money: While it may be tempting to try to piece together your own ELISA to save a few dollars, most researchers find that the cost-benefit analysis doesn't actually add up in the end. After considering all of the different components that must be purchased to generate an assay de novo it is often just as cost effective to buy a pre-made kit.

ELISA Kits - Frequently asked Questions

• What is an ELISA Kit?
• Where can I find protocols and datasheets for the ELISA kits?
• Can I modify the protocol?
• How should I store my samples?
• Can components from different kits be used?
• How many samples can I test on one ELISA?
• Can antibodies-online ELISA Kits be used for diagnostic or therapeutic procedures?
• Do you also offer ELISA Kits with uncoated plates?
• Do your ELISA Kits work for cell and tissue lysates?
• What is the "Independent Validation Initiative"?

What is an ELISA Kit?

ELISA kits are ready-to-use immunoassay kits that contain all the reagents necessary to perform an ELISA (Enzyme-Linked Immunosorbent Assay). ELISA is a plate-based experiment used to detect and quantify molecules such as proteins, antibodies, hormones, peptides, or small molecules like coenzymes, vitamins and so on. Antibodies linked to enzymes are used to quantify the results. There are several formats. The principle of antibody-antigen interaction is different for each format.

Where can I find protocols and datasheets for the ELISA kits?

The manuals, datasheets and MSDS documents for our ELISA kits are either linked on our product detail pages or available on request. The documents contain a detailed protocol, a list of kit components and further information. ELISA kits are characterised by a particularly simple and convenient application, allowing use even by those who are not very familiar with ELISAs. Should any questions or problems arise, you can always contact our customer service, which also consists of biologists with PhDs. With antibodies-online.com you are on the safe side when it comes to ordering high-quality ELISA kits.

Can I modify the protocol?

ELISA Kits have been optimized to provide the best possible results. If you modify the format or protocol, it may give inaccurate and wrong results.

How should I store my samples?

For best results using fresh samples is recommended. Alternatively samples should be stored in a freezer at -20 °C, -80 °C, or in liquid nitrogen.

Can components from different kits be used?

It is not recommended to use components other than those included in the ELISA Kit. The components of a kit are precisely matched to each other and confirmed in their efficacy.

How many samples can I test on one ELISA?

On each 96-well plate an 8-point standard curve with duplicate wells is performed. The remaining wells can be used for samples. If the entire plate is used in an experiment, the remaining 80 wells can accommodate 40 duplicate samples.

Can antibodies-online ELISA Kits be used for diagnostic or therapeutic procedures?

No, the ELISA Kits are for research use only.

Do you also offer ELISA Kits with uncoated plates?

Yes, we do. You can find them using our search filter "plate".

Do your ELISA Kits work for cell and tissue lysates?

The sample types for which an ELISA Kit has been proven to work are indicated in the respective product description.

What is the "Independent Validation Initiative"?

Our Independent Validation Initiative addresses the call for more reproducibility in the proteomics market by providing well documented, independently generated, and scientifically sound data for our ELISA Kits, and other products from our catalog. Please let us know, if you are interested in validating a specific product. In return for your data, you will receive your money back for the product.

About ELISA (Enzyme-linked Immunosorbent Assay)

The enzyme-linked immunosorbent assay, better known as ELISA, is a remarkably versatile and widely used analytical biochemistry technique that was first introduced in the early 1970s. This method enables the quantitative and qualitative determination of antibodies or antigens in a sample and has since established itself as an indispensable tool in laboratories around the world.

The fundamentals of ELISA are based on the antigen-antibody interaction, which is specific and high affinity, meaning that antibodies are able to recognize and bind specific antigens with great precision. This property makes them ideal tools for recognizing molecules in a variety of samples, including blood, serum and other body fluids.

The first publication describing a prototype ELISA procedure came from Peter Perlmann and Eva Engvall at Stockholm University in 1971. At almost the same time, a team of researchers led by Anton Schuurs and Bauke van Weemen developed a similar technique. Both teams used enzyme labelling as an innovative element to visualize the presence of the antigen or antibody. This was a significant advance over the previous methods, which used radioactive isotopes and posed both potential health risks and a shorter shelf life.

Over the years, ELISA has evolved to include several variants, including direct, indirect, sandwich and competitive assays, which can be customized depending on the application and target. This diversity of methods has helped ELISA to be applied in many areas from the diagnosis of infectious diseases and autoimmune disorders to the detection of allergens and drug abuse.

ELISA is characterized by its high sensitivity and specificity and can also be configured for high-throughput screening, making it an indispensable tool in modern medical research and diagnostics. The technique has also contributed significantly to vaccine research and immune response monitoring.

Despite its long history, the ELISA remains a central pillar in life science laboratories due to its flexibility and adaptability to new research needs and disease patterns. Its ongoing development and refinement ensure that the ELISA will continue to play a key role in biomedical research in the future.