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GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting

Name: GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting
SKU: ABIN965412
Price: 599 USD
Availability: InStock

Rockland kca215 DB, WB

Western Blot of anti-GFP monoclonal antibody. (GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting)

1 image

Catalog No. ABIN965412

Supplier Product No.: kca215

Datasheet as PDF

Application

Dot Blot (DB), Western Blotting (WB)

Supplier Product No.

kca215

Supplier

Rockland

Cross-Reactivity

recombinant GFP (rGFP), enhanced GFP (eGFP)
Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 1, beta (IL1B) ELISA Kit
IL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

High Mobility Group Box 1 (HMGB1) ELISA Kit
HMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum

Amyloid beta 1-40 (Abeta 1-40) ELISA Kit
Abeta 1-40 Reactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

Amyloid beta 1-42 (Abeta 1-42) ELISA Kit
Abeta 1-42 Reactivity: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate

Lipopolysaccharides (LPS) ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

High Mobility Group Box 1 (HMGB1) ELISA Kit
HMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
VCAM1 Reactivity: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate

Application Notes

GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Comment

Detection Kit Type: Chemiluminescent Western Blot Kit

Restrictions

For Research Use only
Handling Advice

Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Storage

RT/4 °C
Background

GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: GFP Western Blotting Kit, GFP Chemiluminescent Kit, Green Fluorescent Protein Antibody Kit, Immunoblotting Kit, GFP, rGFP, eGFP