NF-kB p65 ELISA Kit

Catalog No. ABIN965407

Product Details NF-kB p65 ELISA Kit

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

Analytical Method: Quantitative

Characteristics: A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).

Application Details

Application Notes: NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).

Comment:

Detection Kit Type: ELISA Kit

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

References for NF-kB p65 ELISA Kit (ABIN965407)

Antognelli, Moretti, Frosini, Puxeddu, Sidoni, Talesa: "Methylglyoxal Acts as a Tumor-Promoting Factor in Anaplastic Thyroid Cancer." in: Cells, Vol. 8, Issue 6, (2019) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF kB (p65) (NFkBP65 Products)

Synonyms: C-Rel ELISA Kit, NFKB3 ELISA Kit, p65 ELISA Kit, NFkB ELISA Kit, c-rel ELISA Kit, zgc:100833 ELISA Kit, Xrel2 ELISA Kit, Xrel3 ELISA Kit, rel ELISA Kit, rel-A ELISA Kit, rel2 ELISA Kit, rel3 ELISA Kit, v-rel ELISA Kit, xrel ELISA Kit, REL proto-oncogene, NF-kB subunit ELISA Kit, RELA proto-oncogene, NF-kB subunit ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog ELISA Kit, REL ELISA Kit, RELA ELISA Kit, Rela ELISA Kit, Rel ELISA Kit, rel ELISA Kit, rel.L ELISA Kit

Background: The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimmer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
Synonyms: NF-kB Transcription Factor Kit, p65 kit, EIA kit, NF-κB (p65) Transcription Factor Assay

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins