Nuclear Factor kappa B p50 (NFkB p50) ELISA Kit
Reactivity: Human
Colorimetric
DNA-Binding ELISA
-
- Target
- Nuclear Factor kappa B p50 (NFkB p50)
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- DNA-Binding ELISA
- Application
- ELISA
- Purpose
- NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
- Analytical Method
- Quantitative
- Characteristics
- A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate. NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
-
-
- Application Notes
- NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
- Comment
-
Detection Kit Type: ELISA Kit
- Plate
- Pre-coated
- Restrictions
- For Research Use only
-
- Storage
- 4 °C/-20 °C
-
- Target
- Nuclear Factor kappa B p50 (NFkB p50)
- Alternative Name
- NF kB (p50)
- Synonyms
- NF-KB1 ELISA Kit, NF-kappaB ELISA Kit, NF-kappaB1 ELISA Kit, p105 ELISA Kit, p50 ELISA Kit, p50/p105 ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 ELISA Kit, Nfkb1 ELISA Kit
- Background
-
The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimmer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
Synonyms: NF-kB Transcription Factor Kit, p50 kit, EIA kit, NF kappaB ELISA Assay
-
You are here:
