Total Antioxidant Capacity ELISA Kit
Reactivity: Chemical
Colorimetric
Competition ELISA
21.9-4378 μmol/L
1 image
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- Target
- Total Antioxidant Capacity
- Reactivity
- Chemical
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 21.9-4378 μmol/L
- Minimum Detection Limit
- 21.9 μmol/L
- Application
- ELISA
- Analytical Method
- Quantitative
- Sensitivity
- 21.9 μmol/L
- Characteristics
- Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.
- Components
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1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water.
2. Sample diluent: 60 mL x 1 bottle, Ready to use.
3. Cu++ solution: 5 mL x 1 bottle, Ready to use.
4. Stop solution: 5 mL x 1 bottle, Ready to use.
5. Microtiter plate: 1 plate - Material not included
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A. A microplate reader (measuring wavelength 490 nm)
B. Pipettes and pipette chips
C. Plastic test tubes
D. Distilled water
E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).
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- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT
- Storage Comment
- Store at room temperature until the expiration date listed on the kit box label.
- Expiry Date
- The expiry date is stated on the label.
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- Target
- Total Antioxidant Capacity
- Alternative Name
- Total Antioxidant Capacity (PAO)
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