DNA Oxidative Damage - Quantitative Kit
Colorimetric
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- Target
- Oxidative DNA Damage
- Detection Method
- Colorimetric
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Oxidative DNA Damage ELISA Kit
Reactivity: Others Colorimetric Competition ELISA Cell Samples, Plasma, Serum, Tissue Samples, Urine
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- Restrictions
- For Research Use only
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- Target
- Oxidative DNA Damage
- Alternative Name
- Oxidative DNA Quantitative
- Background
- The Oxidative DNA Damage Kit, Quantitative is for the determination of abasic sites in sample DNA, which correspond to colorimetric 96-well microplate assay. ARP standard DNA solutions in this kit are prepared by heat / acid depurination of calf thymus DNA to control the number of abasic sites from 0 to 40 per 1x10 5 bp. These DNA solutions are treated with ARP and purified. The Standard 0 ARP-DNA is prepared using methoxyamine-treated calf thymus DNA without heat/ acid depurination. Since this kit contains centrifugal filtration tubes for the purification of ARP-labeled sample DNA, there is no need to determine the DNA concentration after the ARP reaction. Standard ARP DNA and purified ARP-treated sample DNA are fixed on the 96 well plate with DNA Binding Solution. Then, the number of AP sites in the sample DNA can be determined by the biotin-avidin-peroxidase assay. Since the standard ARP DNA are double GENERAL.Purification of genomic DNA: Several different methods and products are available for the isolation of genomic DNA from samples such as membrane binding method, guanidine/detergent lysis method, and polyelectrolyte precipitation method. Among these methods, the guanidine/detergent lysis method is simple, and it gives highly purified genomic DNA for the ARP-based abasic sites detection.
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