Anti-Nuclear Antibody (ANA) ELISA Kit

Catalog No. ABIN930725

Product Details

Target: Anti-Nuclear Antibody (ANA)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of ANA in the research laboratory
Alternative Names: Anti-nuclear antibodies ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Purified antigens SSA/Ro, SSB/La, RNP 70, Sm, RNP/Sm, centromere B and Jo1 are bound tomicrowells in row A to H separately. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Anti-Nuclear Antibody (ANA)

Alternative Name: anti-ANA (ANA Products)

Target Type: Antibody, Antibody

Background: Inflammatory connective tissue diseases are characterized by idiopathic genesis along with disturbances in terms of cellular and humoral immunity, systemic organ failure and a chronic course of disease. Additionally, connective tissue diseases exhibit overlapping symptomatic features that render an accurate diagnosis difficult. Considering the diversity of mixed connective tissue diseases, such disorders exhibit a common serological characteristic, the presence of antinuclear antibodies. These antibodies are directed against parts of the cell nucleus and the cytoplasm, and many rheumatic diseases are characterized by the presence of one or more of these ANAs. Antibodies to doublestranded DNA (dsDNA), singlestranded DNA (ssDNA), histone, nuclear ribonucleoprotein (RNP) and Smith antigen (Sm) are associated with SLE, while antibodies to Sjogren´s Syndrome A (SSA/Ro) and Sjogren´s Syndrome B (SSB/La) can occur in both SLE and Sjogren´s Syndrome (SS). Antibodies to Jo1 may be observed in polymyositis and dermatomyositis, while antibodies to sclerodermaassociated antigen (Scl70) and centromere can occur in patients with progressive systemic sclerosis (PSS). Antihistone antibodies are associated with SLE and druginduced lupus, while antiRNP antibodies are linked with mixed connective tissue disease (MCTD) and with SLE. Antibodies directed against centromere are associated with CREST syndrome.
Synonyms: Anti-nuclear antibodies ELISA kit.