Anti-Gliadin IgA ELISA Kit
Reactivity: Wheat
Colorimetric
Sandwich ELISA
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- Target
- Anti-Gliadin IgA
- Reactivity
- Wheat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of Gliadin IgA in the research laboratory
Alternative Names: Gliadin IgA ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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AntiGliadin IgA is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgA class antibodies directed against Gliadin. Preparations of purified Gliadin from wheat are coated on the microplates. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96determinations. Each well can be separated from the module (
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Anti-Gliadin IgA
- Target Type
- Antibody
- Background
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Gliadins are proteins containing high amounts of the amino acids prolin and glutamine. This protein belongs to the nutritive tissue of the grain seeds of wheat, oat, barley and rye and is responsible for the baking properties of the flour. Due to the possibilities of the highly specific and sensitive serological determination of IgA and IgG antibodies against gliadin the invasive procedures of biopsies can be given up. In the past several biopsies have been done with patients when celiac disease was suspected, after a period of a gluten-free diet and also after a specific gluten challenge Gliadin antibodies titer correlate very well with the morphological appearance of the mucosa of the upper intestine. It has been well documented that gliadin antibodies level fall very quickly after a gluten free diet has begun and rise immediately after restoring gluten to the diet. Thus the serological test represents a reliable method to monitor patients, and in particular children and teenagers, for their adherence to the glutenfree diet.
Synonyms: Gliadin IgA ELISA kit.
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