Nucleosomes ELISA Kit
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- Target
- Nucleosomes
- Reactivity
- Various Species
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Analytical Method
- Qualitative and Semi-Quantitative
- Characteristics
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ELISA kit for the detection of Nucleosome in the research laboratory
Alternative Names: Nucleosome ELISA kit - Purification
- Immunoaffinity chromatography
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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AntiNucleosome is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against Nucleosome. The assay is based on microplates coated with human Nucleosomes. The microplate can be divided into 12 modules of 8 wells each or can be used complete for 96 determinations. During this procedure the binding of present autoantibodies, as well as the formation of thesandwich complexes and enzymatic color reaction take place during three different reaction phases. The amount of colour is directly proportional to the concentration of IgG present in the original sample. The optical density of the controls serve as reference for the determination of the cutoff OD. Patent results may be classified in terms of negative, positive or strong positive. Alternatively the concentrations of the controls may be used to calculate a calibration curve for semiquantitative results.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Nucleosomes
- Background
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Antibodies directed against nucleosomes were first described in association with systemic lupus erythematosus (SLE) in 1957. At those times known as "LE cell factor". In 1986 nucleosomes were suggested to possibly be important antigens in generating antinuclear antibodies. But only in 1995 nucleosomes were properly described as autoantigens in systemic autoimmune diseases. Today, anti-nucleosome antibodies are recognised in association with SLE. Nucleosomes mainly consist of an octamere of histones (four homodimers of H2A, H2B, H3, H4) around which 146 bp of DNA are wound twice. Histone H1 interacts with the nucleosome and together with linked-DNA connects neighbouring nucleosomes. Hence the nucleosome structure is important in the compaction of DNA in the nucleus.
Synonyms: Nucleosome ELISA kit.
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