BPI ELISA Kit

Catalog No. ABIN930709

Product Details BPI ELISA Kit

Target: BPI (Bactericidal/Permeability Increasing Protein (BPI))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of BPI in the research laboratory
Alternative Names: Bactericidal permeability-increasing protein ELISA kit, BPI ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Highly purified BPI is bound to microwells . Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for BPI

Target: BPI (Bactericidal/Permeability Increasing Protein (BPI))

Alternative Name: anti-BPI (BPI Products)

Target Type: Antibody

Background: The main antigen for the cANCA is the proteinase 3 (PR3), which is a serine proteinase of the present in primary granules. Antibodies of pANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin G and also lysozyme often result in a similar pANCA pattern. Beside different untypical variants of pANCA IF patterns granulocyte specific antinuclear antibodies (GSANA) is indistinguishable from pANCA. This makes a clear interpretation and classification of the IF patterns difficult. Therefore every positive IFANCA findings especially pANCA should be differenciated by ELISA techniques using purified antigens. A survey of documented clinical indications of specific ANCA is given in the table below. PR3ANCA and MPOANCA are reliable serologic markers in the diagnostics of vasculitides. PR3ANCA is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95 %. cANCA is documented to be present in different diseases. The target antigen myeloperoxidase is mainly present (75 %) in microscopic polyangiitis.
Synonyms: Bactericidal permeability-increasing protein ELISA kit, BPI ELISA kit.