Myeloperoxidase ELISA Kit

Catalog No. ABIN930701

Product Details Myeloperoxidase ELISA Kit

Target: Myeloperoxidase (MPO)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of MPO in the research laboratory
Alternative Names: Myeloperoxidase ELISA kit, MPO ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Highly purified myeloperoxidase (MPO) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO (MPO Products)

Target Type: Disease

Background: Anti-neutrophilic cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (cANCA) and a perinuclear (pANCA) type. The main antigen for the cANCA is the proteinase 3 (PR3), which is a serine proteinase of the present in primary granules. Antibodies of pANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin G and also lysozyme often result in a similar pANCA pattern. Beside different untypical variants of pANCA IF patterns granulocyte specific antinuclear antibodies (GSANA) is indistinguishable from pANCA. This makes a clear interpretation and classification of the IF patterns difficult. Therefore every positive IFANCA finding esp. pANCA should be differenciated by ELISA techniques using purified antigens. PR3ANCA and MPOANCA are reliable serologic markers in the diagnostics of vasculitides. PR3ANCA is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95 %. cANCA is documented to be present in different diseases. The target antigen myeloperoxidase is mainly present (75 %) in microscopic polyangiitis.
Synonyms: Myeloperoxidase ELISA kit, MPO ELISA kit.

Pathways: Chromatin Binding