MYH11 ELISA Kit

Catalog No. ABIN930691

Product Details MYH11 ELISA Kit

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Sm1 in the research laboratory
Alternative Names: Smith antigen ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Highly purified RNP/Sm is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: Sm1 (MYH11 Products)

Background: Today the best investigated immunoreactive antigens are double stranded DNA (dsDNA), single stranded DNA, snRNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SSA (Ro) and SSB (La). Smith Antigen (Sm) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE).
Synonyms: Smith antigen ELISA kit.