Thyroperoxidase ELISA Kit

Catalog No. ABIN930685

Product Details Thyroperoxidase ELISA Kit

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Thyroid peroxidase in the research laboratory
Alternative Names: Thyroid peroxidase ELISA kit, TPO ELISA kit, MSA ELISA kit, TPX ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Highly purified human thyroid peroxidase (TPO) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Thyroperoxidase

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Alternative Name: Thyroid peroxidase (TPO Products)

Background: Thyroid peroxidase or thyroperoxidase (TPO) is an enzyme expressed mainly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine (T4) or triiodothyronine (T3), thyroid hormones. In humans, thyroperoxidase is encoded by the TPO gene.
Synonyms: Thyroid peroxidase ELISA kit, TPO ELISA kit, MSA ELISA kit, TPX ELISA kit.

Pathways: Thyroid Hormone Synthesis