Glutamate ELISA Kit
Reactivity: Chemical
Competition ELISA
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- Target See all Glutamate products
- Glutamate
- Reactivity
- Chemical
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Glutamate in the research laboratory
Alternative Names: Glutamate ELISA kit
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Glutamate Assay Kit
BCA Food, Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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After extraction and derivatization Glutamate is determined by ELISA methodology. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. Thereaction is monitored at 450 nm.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target See all Glutamate products
- Glutamate
- Abstract
- Glutamate Products
- Target Type
- Amino Acid
- Background
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Glutamic acid (abbreviated as Glu or E) is one of the 20 proteinogenic amino acids, and its codons are GAA and GAG. It is a nonessential amino acid. The carboxylate anions and salts of glutamic acid are known as glutamates. In neuroscience, glutamate is an important neurotransmitter that plays a key role in long-term potentiation and is important for learning and memory. Glutamate is a key molecule in cellular metabolism. In humans, dietary proteins are broken down by digestion into amino acids, which serve as metabolic fuel for other functional roles in the body. A key process in amino acid degradation is transamination, in which the amino group of an amino acid is transferred to an alpha-ketoacid, typically catalysed by a transaminase.
Synonyms: Glutamate ELISA kit.
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