Histamine ELISA Kit

Catalog No. ABIN930654

Product Details Histamine ELISA Kit

Target: Histamine (HIS)

Reactivity: Rat

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Rat Histamine in the research laboratory
Alternative Names: Rat Histamine ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Assay Procedure:

During the sample preparation Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Histamine

Target: Histamine (HIS)

Alternative Name: Histamine (HIS Products)

Target Type: Chemical

Background: Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Rat Histamine ELISA kit.