Corticosterone ELISA Kit

Catalog No. ABIN930568

Product Details Corticosterone ELISA Kit

Target: Corticosterone (CORT)

Reactivity: Mouse

Method Type: Competition ELISA

Application: ELISA

Cross-Reactivity: Rat (Rattus)

Characteristics: ELISA kit for the detection of Rat/Mouse Corticosterone in the research laboratory
Alternative Names: Rat/Mouse Corticosterone ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This Corticosterone rat/mouse ELISA Kit is a solid phase enzymelinked immunosorbent assay (ELISA), based on the principle of competitive binding. An unknown amount of corticosterone present in the sample and a defined amount of corticosterone conjugated to horseradish peroxidase compete for the binding sites of corticosterone antiserum coated to the wells of a microplate. After incubation on a shaker the microplate is washed four times. After addition of the substrate solution the concentration of corticosterone is inversely proportional to the optical density measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Corticosterone

Target: Corticosterone (CORT)

Alternative Name: Corticosterone (CORT Products)

Target Type: Hormone

Background: Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone is a major indicator of stress since stress increases the production of corticosteroids. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
Synonyms: Rat/Mouse Corticosterone ELISA kit.