(1 reference)-
- Target See all Testosterone ELISA Kits
- Testosterone
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Reactivity
- Rat
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Testosterone in the research laboratory
Alternative Names: Rat/Mouse Testosterone ELISA kit - Top Product
- Discover our top product Testosterone ELISA Kit
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Testosterone ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Plasma, Serum
Testosterone ELISA KitReactivity: Rat Colorimetric Competition ELISA 0.13-25.6 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Testosterone ELISA KitReactivity: Various Species Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Plasma, Saliva, Serum, Urine
Testosterone ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Testosterone ELISA KitSmall Sample Reactivity: Various Species Colorimetric Competition ELISA 370.4 pg/mL - 30000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Testosterone ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Testosterone ELISA KitReactivity: Pig Colorimetric Competition ELISA 0.1-20 ng/mL Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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This Testosterone rat/mouse ELISA Kit is a solid phase enzymelinked immunosorbent assay (ELISA), based on the principle of competitive binding. An unknown amount of testosterone present in the sample and a defined amount of testosterone conjugated to horseradish peroxidase compete for the binding sites of testosterone antiserum coated to the wells of a microplate. After onehour incubation on a shaker the microplate is washed four times. After addition of the substrate solution the concentration of testosterone is inversely proportional to the optical density measured.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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: "Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats." in: Nutrition & metabolism, Vol. 5, pp. 31, (2009) (PubMed).
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: "Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats." in: Nutrition & metabolism, Vol. 5, pp. 31, (2009) (PubMed).
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- Target See all Testosterone ELISA Kits
- Testosterone
- Abstract
- Testosterone Products
- Target Type
- Hormone
- Background
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Testosterone is a steroid hormone from the androgen group synthesized by the Leydig cells in the testes in males, the ovaries in females, and adrenal glands in both sexes. It exerts a wide-ranging influence over sexual behaviour, muscle mass and strength, energy, cardiovascular health and bone integrity. Testosterone biosynthesis coincides with the spermatogenesis and fetal Leydig cell differentiation in the male rat. Several in vivo models including hormone-suppression, hormone-restoration and hypophysectomy were established for the study of the hormonal regulation of spermatogenesis by testosterone. In the Brown Norway rat, serum testosterone levels decrease with aging, accompanied by increases in serum FSH. The capacity of Leydig cells to produce testosterone is higher in young than in old rats. Testosterone secreted during late gestational and neonatal periods causes significant brain sexual dimorphism in the rat. This results in both sex-specific behaviour and endocrinology in adults. Analyses concerning the regulation of synthesis reveal that testosterone is able to regulate its own synthesis and indicate that this autoregulation is the result of rapid, specific inhibition by testosterone of 17 alpha-hydroxylase activity.
Synonyms: Rat/Mouse Testosterone ELISA kit.
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