Anti-Sperm Antibody (AsAb) ELISA Kit
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- Target
- Anti-Sperm Antibody (AsAb)
- Reactivity
- Human
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Sperm Antibodies in the research laboratory
Alternative Names: Sperm antibodies ELISA kit
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Anti-Sperm Antibody (AsAb) ELISA Kit
AsAb Reactivity: Human Colorimetric Competition ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
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- Application Notes
- Optimal conditions to be determined by end-user
- Assay Procedure
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The antispermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from IBLAmerica is a solidphasesandwich enzymeimmunoassay for the quantitative determination of antispermatozoa antibodies in human seminal plasma. The ELISAplate is coated with a mix of spermatozoa proteins which are recognized by antispermatozoaantibodies. The samples and standards are pipetted into the wells and then incubated. During this incubationantispermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. Afterwashing the enzyme conjugate, consisting of antihuman globulin antibodies covalently coupled tohorseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradishperoxidase oxidizes the then added substrate TMB (3,3',5,5'tetramethylbenzidine) yielding a colour reactionwhich is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nmwith a microplate reader. The use of a reference measurement with a wavelength of 550 nm or higher is recommended.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Anti-Sperm Antibody (AsAb)
- Target Type
- Antibody
- Background
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Antibodies directed against spermatozoa antigens may cause infertility in women or men. Antispermatozoa antibodies exert heterogeneous effects on the ability of spermatozoa to fertilize. The inhibiting effect of antispermatozoa antibodies on the motility of spermatozoa by binding to their surface and by agglutinating processes is wellknown. The penetration of the spermatozoa into the cervical mucus is impaired by the presence of antispermatozoa antibodies in the seminal plasma and/or in the cervical mucus. Antispermatozoa antibodies negatively influence the capacitation and the acrosome reaction of spermatozoa and thereby impede the interaction of the spermatozoa with the oocyte. The interaction of the spermatozoon with the oocyte and the subsequent binding to and penetration of the zona pellucida may be inhibited by antispermatozoa antibodies. The following fusion of the oocyte and a spermatozoon may also be impaired by the presence of antispermatozoa antibodies.
Synonyms: Sperm antibodies ELISA kit.
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