Estrone ELISA Kit
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- Target See all Estrone ELISA Kits
- Estrone
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Reactivity
- Hormone
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Estrone in the research laboratory
Alternative Names: Estrone ELISA kit - Purification
- Antiserum
- Top Product
- Discover our top product Estrone ELISA Kit
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Estrone ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 125 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrone ELISA KitReactivity: Rat Colorimetric Competition ELISA 0.156 pmol/mL - 10 pmol/mL Plasma, Serum, Tissue Homogenate
Estrone ELISA KitSmall Sample Reactivity: Various Species Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrone ELISA KitReactivity: Various Species Colorimetric Sandwich ELISA Fecal, Tissue Culture Medium, Urine
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competitionoccurs between an unlabeled antigen (present in standards, control and patient samples) and an enzymelabelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. Theintensity of the colour formed is inversely proportional to the concentration of estrone in the sample. A set of standards is used to plot a standard curve from which the amount of estrone in patient samples and controls can be directly read.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target See all Estrone ELISA Kits
- Estrone
- Abstract
- Estrone Products
- Target Type
- Amino Acid
- Background
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Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the estrone level shows a slight increase. The production of estrone then increases markedly to peak at around day 13. The peak is of short duration and by day 16 of the cycle levels will be low. A second peak occurs at around day 21 of the cycle and if fertilization does not occur, then the production of estrone decreases.
Synonyms: Estrone ELISA kit.
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