Toxoplasma IgA ELISA Kit
Reactivity: Toxoplasma gondii
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- Target
- Toxoplasma IgA (Toxoplasma IgA Antibody)
- Reactivity
- Toxoplasma gondii
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Toxoplasma gondii IgA in the research laboratory
Alternative Names: Toxoplasma gondii IgA ELISA kit
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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Diluted serum is added to wells coated with purified Toxoplasma antigen. Toxoplasma IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibodyantigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Toxoplasma IgA (Toxoplasma IgA Antibody)
- Target Type
- Antibody
- Background
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Toxoplasma gondii causes toxoplasmosis, a common disease that affects 30-50 of every 100 people in North America by the time they are adults. The mean source of infection is direct contact with cat feces or from eating undercooked meats. Toxoplasmosis generally presents with mild symptoms in immunocompetent individuals, in the immunocompromised patient, however, the infection can have serious consequences. Acute toxoplasmosis in pregnant women can result in miscarriage, poor growth, early delivery or stillbirth. Treatment of an infected pregnant woman may prevent or lessen the disease in her unborn child. Treatment of an infected infant will also lessen the severity of the disease as the child grows. IgG and IgM antibodies to Toxoplasma can be detected with 23 weeks after exposure. IgG remains positive, but the antibody level drops over time. ELISA can detect Toxoplasma IgM antibody one year after infection in over 50 % of patients. Therefore, IgM positive results should be evaluated further with one or two follow up samples if primary infection is suspected.
Synonyms: Toxoplasma gondii IgA ELISA kit.
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