MUC1 ELISA Kit

Catalog No. ABIN930404

Product Details MUC1 ELISA Kit

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of CA 15-3 in the research laboratory
Alternative Names: CA 15-3 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

The CA 15-3 ELISA test is based on the principle of a solid phase enzymelinked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA 15-3 molecule is used for solid phase immobilization (on the microtiter wells). A rabbit anti CA 15-3 antibody conjugated to horseradish peroxidase (HRPO) is in the antibodyenzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA 15-3 molecules being sandwiched between the solid phase and enzymelinked antibodies. After two separate 1 hour incubation steps at 37 °C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA 15-3 is directly proportional to the color intensity of the test sample. Absorbance ismeasured spectrophotometrically at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: CA 15-3 (MUC1 Products)

Target Type: Disease

Background: Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year. Roughly half of these newly diagnosed patients are node-negative, however 30 % of these cases progress to metastatic disease. There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent S phase profile, epidermal growth factor receptor, HER2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA 15-3. CA 15-3 is most useful for monitoring patients post-operatively for recurrence, particularly of metastatic diseases. 90 % of patients with local and systemic recurrence have elevated CA 15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 20 % increase in the serum CA 15-3 is associated with progression of carcinoma. A 50 % decrease in serum CA153 is associated with response to treatment. CA 15-3 is more sensitive than CEA in early detection of breast cancer recurrence. In combination with CA 12-5, CA 15-3 has been shown to be useful in early detection of relapse of ovarian cancer. CA 15-3 levels are also increased in colon, lung and hepatic tumors.
Synonyms: CA 15-3 ELISA kit.

Pathways: Negative Regulation of intrinsic apoptotic Signaling