Triiodothyronine T3 ELISA Kit

Catalog No. ABIN930403

Product Details Triiodothyronine T3 ELISA Kit

Target: Triiodothyronine T3 (T3)

Reactivity: Hormone

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Triiodothyronine Uptake in the research laboratory
Alternative Names: Triiodothyronine ELISA kit, T3 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The required components for assessing the binding capacity of human serum are enzymeT3 conjugate, thyroxine, binding protein (P), and immobilized thyroxine antibody (Ab). Upon mixing the enzymeconjugate and thyroxine with the specimen, a binding reaction results between the patient's binding proteins and the added thyroxine but not with the enzyme conjugate. The added thyroxine (T4) not consumed in reaction 1 then competes with the enzymeT3 conjugate for a limited number of insolubulized binding sites. After equilibrium is attained, the antibodybound fraction is separated from unbound enzymeantigen by decantation or aspiration. The enzyme activity in theantibodybound fraction is directly proportional to the binding capacity of the specimen. Thus, in hypothyroidism, the binding proteins are relatively unsaturated (due to the low level of thyroid hormones) resulting in higher consumption of the added thyroxine than a euthyroid specimen. This leads to higher binding of the enzymetriiodothyronineconjugate caused by the reduced concentration of the available thyroxine. In hyperthyroidism, the reverse is true. The binding proteins are relatively saturated with thyroxine (due to the high level of thyroid hormone) resulting in lower consumption of the added thyroxine. The remaining thyroxine is relatively much higher than an euthyroid specimen resulting in lower enzymeantigen antibody binding due to the increased competition of the thyroxine for the limited antibody sites.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Alternative Name: Triiodothyronine (T3 Products)

Target Type: Amino Acid

Background: The thyroid gland under the regulatory control of thyrotropin hormone secretes thyroxine (T4) and triiodothyronine (T3) into the general circulation. The released hormones do not circulate as free molecules but are almost entirely (99.9 %) bound to specific hormone binding serum proteins. Three protein fractions with varying affinities and capacities for interaction with T3 and T4 have been identified by reverse flow paper electrophoresis. Thyroxine binding globulin (TBG) carries 65-79 % of the total circulating concentration. Thyroxine binding prealbumin (TBPA) has an intermediate avidity for thyroxine (carries approximately 15-29 %) but little if any avidity for triiodothyronine. Albumin with a low affinity but high capacity carries 19 % of thyroxine and 39 % of the available triiodothyronine. Since the metabolic processes are regulated entirely by the concentration of the free thyroid hormones, which are inversely related to the levels of the binding proteins, an assessment of the binding capacity of human serum was developed in 1957 by Hamolsky. In this early method, radioactive T3 was added to a specimen of whole blood. After an incubation period, the mixture was centrifuged and the red cells washed. The radioactivity uptake of the red cells was inversely related to the binding capacity of the serum. Although this method had severe limitations, it proved to be a valuable diagnostic tool.
Synonyms: Triiodothyronine ELISA kit, T3 ELISA kit.