Substance P ELISA Kit
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- Target See all Substance P ELISA Kits
- Substance P
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Reactivity
- Human
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Substance P in the research laboratory
Alternative Names: Substance P ELISA kit - Top Product
- Discover our top product Substance P ELISA Kit
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Substance P ELISA Kit
Reactivity: Rat Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Rat Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Substance P ELISA KitReactivity: Dog Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Human Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Mouse Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Substance P ELISA KitReactivity: Guinea Pig Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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This Substance P kit is a competitive immunoassay for the determination of Substance P in biological fluids. Please read the complete kit insert before performing this assay. The kit for the measurement of Substance P uses a polyclonal antibody to Substance P to bind, in a competitive manner, the Substance P in the standard or sample or an alkalinephosphatase molecule which has Substance P covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Substance P in either standards or samples. The measured optical density is used to calculate the concentration of Substance P.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target See all Substance P ELISA Kits
- Substance P
- Abstract
- Substance P Products
- Background
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Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman's group in 1970. Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.
Synonyms: Substance P ELISA kit.
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