HSPD1 ELISA Kit

Catalog No. ABIN930390

Product Details HSPD1 ELISA Kit

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human

Method Type: Sandwich ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Hsp60 in the research laboratory
Alternative Names: Human Hsp60 ELISA kit, HLD4 ELISA kit, SPG13 ELISA kit, Chaperonin 60 ELISA kit, HSP65 ELISA kit, Heat Shock Protein 65 ELISA kit, GROEL ELISA kit

Purification: Protein G affinity column

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

HSP60 (human), EIA is a sandwich immunoassay. A mouse monoclonal antibody specific for Hsp60 is precoated on thewells of the provided AntiHsp60 Immunoassay Plate. Hsp60 is captured by the immobilized antibody and is detected with an Hsp60 specific, goat polyclonal antibody. The goat polyclonal antibody is subsequently bound by a horseradish peroxidase conjugated antigoat IgG secondary antibody. The assay is developed with tetramethylbenzidine substrate and a blue color develops in proportion to the amount of captured Hsp60. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450nm. Hsp60 concentrations from the sample are determined by interpolating absorbance readings from a standard curve generated with the calibrated Hsp60 protein standard provided.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: Hsp60 (HSPD1 Products)

Background: Heat shock protein 60 (Hsp60) is a molecular chaperone that participates in the folding of mitochondrial proteins and facilitates proteolytic degradation of misfolded or denatured proteins. Hsp60 is strongly dependent upon its co-chaperone, Hsp10, which binds the molecule and regulates its substrate binding and ATPase activity. Hsp60 is rapidly upregulated by a range of cellular insults including oxidative stress, viral infection, chemical exposure, increased temperature, irradiation etc. Hsp60 is mainly located in the mitochondria but changes in the intracellular location and cell surface expression have been reported. The Hsp60 family of proteins, which includes GroEL from E. coli and Hsp65 from mycobacteria, are highly conserved through evolution and an autoimmune reaction may be evoked because of the immunological cross reaction between self Hsp60 and microbial pathogens. Human Hsp60 also shares sequence homology with a wide range of autoantigens and the extent of this homology may contribute to autoimmunity. Autologous Hsp60, when expressed or released from stressed autologous cells, may serve as a danger signal for the innate immune system to mount an adaptive response. Both B and T cell responses to autologous Hsp60 have been reported to occur in a variety of inflammatory conditions. Anti Hsp60 antibody levels have been studied in the peripheral circulation of normal controls and in the aging process.
Synonyms: Human Hsp60 ELISA kit, HLD4 ELISA kit, SPG13 ELISA kit, Chaperonin 60 ELISA kit, HSP65 ELISA kit, Heat Shock Protein 65 ELISA kit, GROEL ELISA kit.

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity