Myeloperoxidase ELISA Kit

Catalog No. ABIN930385

Product Details Myeloperoxidase ELISA Kit

Target: Myeloperoxidase (MPO)

Reactivity: Human

Application: ELISA

Characteristics: ELISA kit for the detection of Human Myeloperoxidase in the research laboratory
Alternative Names: Human Myeloperoxidase ELISA kit, MPO ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The IBLAmerica human Myeloperoxidase (MPO) Enzyme Immunometric Assay (EIA) kit is complete kit for the determination ofMPO in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to MPO immobilized on amicrotiter plate to bind theMPO in the standards or sample. A native MPO Standard is provided in the kit. After a short incubation, the excess sample or standard is washed out and a rabbit polyclonal antibody to MPO is added. This antibody binds to the MPO captured on the plate. After a short incubation, the excess antibody is washed out and goat antirabbit IgG conjugated to horseradish peroxidase is added, which binds to the polyclonal MPO antibody. Excess conjugate is washed out and substrate is added.After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of MPO in either standards or samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase (MPO Products)

Background: Myeloperoxidase (MPO) is synthesized as a single chain precursor, which is then processed into a tetramer consisting of 2 light chains and 2 heavy chains. In response to microbial invasion, MPO is released from the cytoplasmic granules of neutrophils into the extracellular space and phagosome, where it generates hypohalous acids that have a strong microbicidal effect. Since myeloperoxidase is a major component of myeloid precursor cells, the enzymatic activity of MPO is used to classify acute leukemias. Myeloperoxidase is used also as a marker of airway inflammation caused either by disease, such as asthma, or environmental irritants. In addition to the protein itself, autoantibodies against MPO are markers for several systemic vasculitis diseases.
Synonyms: Human Myeloperoxidase ELISA kit, MPO ELISA kit.

Pathways: Chromatin Binding