BAX ELISA Kit

Catalog No. ABIN930384

Product Details BAX ELISA Kit

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human

Application: ELISA

Characteristics: ELISA kit for the detection of Human Bax in the research laboratory
Alternative Names: Human Bax ELISA kit, BCL24 ELISA kit, BCL2-associated X protein ELISA kit, Bcl2-L-4 ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This human Bax Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of Bax in human cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to Bax immobilized on a microtiter plate to bind the Bax in the standards or sample. A recombinant human Baxalpha Standard is provided in the kit. After a short incubation, the excess sample or standard iswashed out and a biotinylated monoclonal antibody to Bax is added. This antibody binds to the Bax captured on the plate. After a short incubation, theexcess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated monoclonal Bax antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of Bax in either standards or samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax (BAX Products)

Background: Many physiological processes, including cellular development and homeostasis, are regulated by a balance between cell proliferation, differentiation and apoptosis. Several gene products play a significant role in regulating apoptosis. The Bcl2 gene family is comprised of both proapoptotic (e.g. Bax, Bid) and antiapoptotic (e.g. Bcl2, BclXL) members, with membership dependent on the presence of at least one Bcl2 homology (BH) domain. These proteins form homo and heterodimers, with the ratio between pro and antiapoptotic levels an important determinant of cell survival. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl2 in vivo. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase 3 and perpetuating the apoptotic cascade 5. Protein levels of Bax and other Bcl2 family members are altered in various human malignancies, with changes in expression levels implicated in diseases involving apoptosis such as cancer, autoimmune diseases and neurodegenerative disorders.
Synonyms: Human Bax ELISA kit, BCL24 ELISA kit, BCL2-associated X protein ELISA kit, Bcl2-L-4 ELISA kit.

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response