XIAP ELISA Kit

Catalog No. ABIN930381

Product Details XIAP ELISA Kit

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivity: Human

Application: ELISA

Characteristics: ELISA kit for the detection of Human XIAP in the research laboratory
Alternative Names: Human XIAP ELISA kit, X-linked Inhibitor of Apoptosis ELISA kit, X-linked IAP ELISA kit, API3 ELISA kit, BIRC4 ELISA kit, ILP1 ELISA kit, IAP3 ELISA kit, IAP-like Protein ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This human XIAP Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of XIAP in human cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to human XIAP immobilized on a microtiter plate to bind the XIAP in the standards or sample. Arecombinant human XIAP Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a biotinylatedmonoclonal antibody to human XIAP is added. This antibody binds to the XIAP captured on the plate. After a short incubation the excess antibody is washed out and streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated monoclonal XIAP antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of human XIAP in either standards or samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP (XIAP Products)

Background: XIAP (X-linked Inhibitor of Apoptosis) is known by a variety of other names (baculoviral IAP repeat containing protein 4 (BIRC4), mammalian IAP homolog A (MIHA), X-linked inhibitor of apoptosis protein 3, Xlinked IAP, HILP, IAP Like protein and ILP). XIAP belongs to the IAP family (inhibitors of apoptosis) which is characterized by one or more BIR (baculovirus IAP repeat) domains. These proteins have multiple biological functions that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. XIAP is a broad ranging suppressor of apoptosis. XIAP functions by direct binding and inhibition of several caspases, including caspase 3, caspase 7 and caspase 9.
Synonyms: Human XIAP ELISA kit, X-linked Inhibitor of Apoptosis ELISA kit, X-linked IAP ELISA kit, API3 ELISA kit, BIRC4 ELISA kit, ILP1 ELISA kit, IAP3 ELISA kit, IAP-like Protein ELISA kit.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis