Gastrin ELISA Kit

Catalog No. ABIN930372

Product Details Gastrin ELISA Kit

Target: Gastrin (GAST)

Reactivity: Rat

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Gastrin in the research laboratory
Alternative Names: Rat Gastrin ELISA kit, CCK ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This rat Gastrin I kit is a competitive immunoassay for the determination of Gastrin in samples. The kit uses a polyclonal antibody to Gastrin I to bind, in a competitive manner, the Gastrin I in the sample or an alkaline phosphatase molecule which has Gastrin I covalently attached to it. After a simultaneous incubation at room temperature the excess reagentsare washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellowcolor generated read on amicroplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Gastrin I in either standards or samples. The measured optical density is used to calculate the concentration of Gastrin I.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Gastrin

Target: Gastrin (GAST)

Alternative Name: Gastrin (GAST Products)

Background: Gastrins are a family of sequence-related carboxy-amidated peptides produced by endocrine G Cells of the antrum mucosa and duodenum in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Gastrin is synthesized as a 104 residue prepropeptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34 and G173. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine 12 residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. G34 is the major circulating Gastrin in fasting serum, but with G17, increases two to three-fold after feeding until both are present in approximately equal amounts. Normal serum levels in rats have been reported over a range of 40169 pg/mL with possible variations between animal sources.
Synonyms: Rat Gastrin ELISA kit, CCK ELISA kit.