IgG1 ELISA Kit

Catalog No. ABIN930364

Product Details IgG1 ELISA Kit

Target: IgG1

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Media, Serum

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Mouse IgG1 Isotyping in the research laboratory
Alternative Names: Mouse IgG1 Isotyping ELISA kit

Purification: Protein A affinity column

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Mouse IgG1 Isotyping ELISA kit

Plate: Pre-coated

Protocol: This mouse IgG1 Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of mouse IgG1 in Tissue Culture Media and serum. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG1 Standard is provided in thekit. After a simultaneous incubation with a polyclonal antibody to mouse IgG1 conjugated to Horseradish peroxidase, which binds to the mouse IgG1 captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. Themeasured optical density is directly proportional to the concentration ofmouse IgG in either standards or samples. - See more at: https://www.fitzgerald-fii.com/mouse-igg1-isotyping-elisa-kit-55r-ib09602.html#sthash.qbaaakZ4.dpuf

Assay Procedure:

This mouse IgG1 Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of mouse IgG1 in Tissue Culture Media and serum.
Please read the complete kit insert before performing this assay.
The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG1 Standard is provided in the kit.
>After a simultaneous incubation with a polyclonal antibody to mouse IgG1 conjugated to Horseradish peroxidase, which binds to the mouse IgG1 captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or samples.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C

Target Details for IgG1

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background: IgG is divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. IgG is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG1 apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta. Synonyms: Mouse IgG1 Isotyping ELISA kit.