IGFBP2 ELISA Kit

Catalog No. ABIN930357

Product Details IGFBP2 ELISA Kit

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Reactivity: Mouse

Application: ELISA

Characteristics: ELISA kit for the detection of Mouse IGFBP2 in the research laboratory
Alternative Names: Mouse IGFBP2 ELISA kit, Insulin-like growth factor Binding protein 2 ELISA kit, BP2 ELISA kit, IGF-binding Protein 2 ELISA kit

Purification: Immunoaffinity chromatography

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This ELISA for IGFBP2 utilizes a specific high affinity polyclonal antibody and a specific monoclonal antibody for this protein. The ELISA recognizes quantitatively mouseIGFBP2 and is unaffected by an excess of IGFl or IGFII levels. Related molecules such as lGFBP3 show no crossreactions in the assay. The standards are prepared of recombinant mouseIGFBP2 in the range of 0.125 to 8 ng/mL. The theoretical sensitivity of the assay is 0.04 ng/mL (2 x SD of zerostandard). Intraassay and interassay variation coefficients were found both < 10 % .

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for IGFBP2

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Alternative Name: IGFBP2 (IGFBP2 Products)

Background: Insulin-like growth factors (IGFs) regulate proliferation, differentation, apoptosis, cell adhesion and metabolism in various tissues and cell types. IGFI, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGFII is mainly a growth factor of fetal tissue. The biological actions of IGF over the IGFTypeI receptor are modulated variably through the IGF binding proteins (IGFBP1 to 6). IGFBP2 is, after IGFBP3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF forms and hormones regulate the expression of IGFBP2, GH effect is thereby inhibiting. At cellular level IGFBP2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism.
Synonyms: Mouse IGFBP2 ELISA kit, Insulin-like growth factor Binding protein 2 ELISA kit, BP2 ELISA kit, IGF-binding Protein 2 ELISA kit.

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation