IGFBP2 ELISA Kit

Catalog No. ABIN930356

Product Details IGFBP2 ELISA Kit

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Reactivity: Human

Application: ELISA

Characteristics: ELISA kit for the detection of IGFBP2 in the research laboratory
Alternative Names: IGFBP2 ELISA kit, Insulin-like growth factor Binding protein 2 ELISA kit, BP2 ELISA kit, IGF-binding Protein 2 ELISA kit

Purification: Immunoaffinity chromatography

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The ELISA for IGFBP2 utilizes a specific high affinity polyclonal antibody and a specific monoclonal antibody for this protein. It recognizes quantitatively IGFBP2 and is unaffected by excess of IGFl or IGFII levels. Related molecules such as lGFBP3 show no crossreaction in the assay. The standards are also prepared of recombinant IGFBP2 in the range of 1 to 80 ng/mL. Detailed specification of antibody and pure IGFBP2 (data sheets) can be ordered from Mediagnost GmbH. Chromatographically purified IGFBP2, as well as IGFBP2antiserum are available at Mediagnost GmbH also as research reagents. Different human sera were spiked with recombinant human IGFBP2:recovery was found between 97 % and 115 % . The theoretical sensitivity of the assay is 0.2 ng/mL (2 x SD of zero standard). Intraassay and interassay variation coefficients were found both < 10 % .

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for IGFBP2

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Alternative Name: IGFBP2 (IGFBP2 Products)

Background: Insulin-like growth factors (IGFs) regulate proliferation, differentation, apoptosis, cell adhesion and metabolism in various tissues and cell types. IGFI, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGFII is mainly a growth factor of fetal tissue. The biological actions of IGF over the IGFTypeI receptor are modulated variably through the IGF binding proteins (IGFBP1 to 6). IGFBP2 is, after IGFBP3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF forms and hormones regulate the expression of IGFBP2, GH effect is thereby inhibiting. At cellular level IGFBP2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism.
Synonyms: IGFBP2 ELISA kit, Insulin-like growth factor Binding protein 2 ELISA kit, BP2 ELISA kit, IGF-binding Protein 2 ELISA kit.

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation