Calcitonin ELISA Kit

Catalog No. ABIN930354

Product Details Calcitonin ELISA Kit

Target: Calcitonin (Calca)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Calcitonin in the research laboratory
Alternative Names: Calcitonin ELISA kit, CT ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

The Calcitonin Immunoassay is a twosite ELISA [EnzymeLinked ImmunoSorbent Assay] for the measurement of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for welldefined regions on the calcitonin molecule. One antibody binds only to Calcitonin 1123 and this antibody is biotinylated. The other antibody binds only to Calcitonin 2132 and this antibody is labeled withhorseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidincoated microplate well.Thus the calcitonin in the sample is sandwiched between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in thesample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and patient samples are determined directly from this curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Calcitonin

Target: Calcitonin (Calca)

Alternative Name: Calcitonin (Calca Products)

Background: Calcitonin (CT) is a 32 amino acid peptide hormone secreted by the parafollicular C cells of the thyroid gland under serum calcium control. After acute administration, this peptide acts as a potent hypocalcemic and hypophosphatemic hormone by increasing renal calcium clearance and reducing bone resorption. However, its precise physiological role in bone metabolism is not yet fully understood. Various forms of CT may be detected in blood samples, including a CT monomer, an oxidized monomer, a dimer, higher molecular weight forms, and possibly precursor of CT. The concentrations of these peptides vary with clinical status, renal function and tissular origin of CT (normal or ectopic production). Medullary thyroid carcinoma (MTC) is a malignant tumor, developed from the C cells, secreting calcitonin in large excess. This disease occurs either as a sporadic (80 %) or a familial (20 %) form, which is transmitted as an autosomal dominant gene or as a component of multiple endocrine neoplasia (IIb). Moderate hypercalcitoninemia is also observed in pregnancy, pernicious anaemia, renal failure and during the neonatal period. Preferably, monomer form of CT is detected in this assay.
Synonyms: Calcitonin ELISA kit, CT ELISA kit.