PTH ELISA Kit

Catalog No. ABIN930353

Product Details PTH ELISA Kit

Target: PTH (Parathyroid Hormone (PTH))

Reactivity: Hormone

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of PTH intact in the research laboratory
Alternative Names: PTH ELISA kit, Parathyroid Hormone ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

The Intact PTH Immunoassay is a twosite ELISA [EnzymeLinked ImmunoSorbent Assay] for the measurement of the biologically intact 84 amino acid chain of PTH. Two different goat polyclonal antibodies to human PTH have been purified by affinity chromatography to be specific for well defined regions on the PTH molecule. One antibody is prepared to bind only the midregion and Cterminal PTH 3 984 and this antibody is biotinylated. The other antibody is prepared to bind only the Nterminal PTH 134 and this antibody is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of intact PTH in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of intact PTH present in the controls and patient samples are determined directly from this curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for PTH

Target: PTH (Parathyroid Hormone (PTH))

Alternative Name: PTH (PTH Products)

Target Type: Hormone

Background: PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a preproparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25 amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypetide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and mid-region fragments. In subjects with renal insufficiency, C-terminal and mid-region PTH assays typically give elevated PTH results, as reflected by impaired renal clearance.
Synonyms: PTH ELISA kit, Parathyroid Hormone ELISA kit.

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process