HCP ELISA Kit
HCP
Reactivity: Pichia pastoris
Colorimetric
Sandwich ELISA
12.5 ng/mL - 800 ng/mL
Cell Culture Supernatant
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- Target See all HCP products
- HCP (Host Cell Proteins (HCP))
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Reactivity
- Pichia pastoris
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 12.5 ng/mL - 800 ng/mL
- Minimum Detection Limit
- 12.5 ng/mL
- Application
- ELISA
- Purpose
- The kit is intended for the quantitative determination of residual host cell protein in Cell Culture Supernatants, Protein Purification Process, and End-Product expressed by Pichia Yeast expression systems.
- Sample Type
- Cell Culture Supernatant
- Analytical Method
- Quantitative
- Cross-Reactivity (Details)
- No cross-reaction exists between the homologous antigens.
- Sensitivity
- 1.56 ng/mL
- Characteristics
- This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
- Components
- Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 µL
- Assay Time
- 3 h
- Plate
- Pre-coated
- Assay Precision
- Intra Variation%: 7-11, Inter Variation%: 1.1-5.8
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all HCP products
- HCP (Host Cell Proteins (HCP))
- Alternative Name
- Host Cell Proteins (HCP Products)
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