plexWell™ 96

Catalog No. ABIN7271572

Product Details

Application: Library Preparation (LibPrep)

Purpose: plexWell™96 Library Preparation Kit for Illumina® Sequencing Platforms

Specificity:

• Kit contains major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
• Efficient library prep for one 96-well plate inputs
• Normalizes input DNA over wide input range of 3-30 ng
• Volume-based pricing (save 30-50 % on total lab costs)

Characteristics: plexWell NGS multiplexed library generation kit for Illumina®

• Assay-ready 96-well fully-skirted low-profile PCR plate
• 2,304 (96 x 24) unique barcode combinations

6 sets of 4 pool barcodes (PB Set A, B, C, D, E, or F)

Components: Box 1:

• Sample Barcode Plate (-20°C)

Box 2:

• 3X Coding Buffer (ambient)
• X Solution (ambient)
• MAGwise™ Paramagnetic Beads (4°C)

Box3:

• Pool Barcode Reagent (-20°C)
• Library Primer Mix (-20°C)

Application Details

Application Notes:

• Large scale full length viral surveillance
• Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc)
• Microbiome screening
• Microbial whole genome sequencing
• scRNA-seq
• Low-depth whole genome/GBS

Comment:

Time-to-prepare a multiplexed library from 96 samples:

• Hands-on time: 50 min
• Total elapsed time: 3 hrs

Recommended input: 3-30 ng of genomic DNA per sample (n=96)
Expected Results:

• Library Yield: 750 - 1500 fmoles of purified multiplexed library
• Library Diversity: <5 % dupe rate per 1M sequenced reads per sample
• Substantial normalization: Typically <2.5-fold range (min to max) in read count across all 96 samples

Compatibility with Illumina sequencing systems: Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Box 1 and Box 3 + cDNA: ship on dry ice, store at -20 °C
Box 2: ship ambient. Do NOT freeze!, store Magwise at 4 °C and Coding Buffer as well as X Solution at 4 °C to 30 °C