CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair

Catalog No. ABIN7035517

Product Details

Target: CD47

Reactivity: Human

Characteristics: This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human CD47 and in-house developed biotinylated SIRP alpha protein. This product is uniquely suitable for rapid high-throughput screening of putative CD47 inhibitors. Briefly, we provide you with a biotinylated human SIRP alpha protein, a human CD47 protein, an anti-CD47 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent. Your experiment will include 4 simple steps:
a) Coat the plate with human CD47.
b) Mix biotinylated human SIRP alpha and your molecule of interest.
c) Add to the coated human CD47.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.

Components:

• Human CD47
• Biotinylated Human SIRP alpha
• Streptavidin-HRP
• Anti-CD47 Neutralizing Antibody

Application Details

Application Notes: This pair is useful for screening for inhibitors of human CD47 binding to human SIRP alpha.

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: No activity loss was observed after storage at: -20°C for 1 year in lyophilized state after receipt, -70°C for 6 months under sterile conditions after reconstitution with carrier protein., Powder,RT

Target Details

Target: CD47

Alternative Name: CD47 (CD47 Products)

Synonyms: IAP Kit, MER6 Kit, OA3 Kit, 9130415E20Rik Kit, AA407862 Kit, AI848868 Kit, AW108519 Kit, B430305P08Rik Kit, Itgp Kit, CD47/IAP Kit, alkaline phosphatase, intestinal Kit, CD47 molecule Kit, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Kit, Cd47 molecule Kit, ALPI Kit, CD47 Kit, Cd47 Kit

Background: Immune checkpoint pathway is a focal point of today’s cancer research. CD47 is one of the best characterized checkpoint proteins. CD47 binding to SIRP alpha aids in tumor evasion of the immune system. Therefore, the pharmaceutical inhibition of CD47 has been considered a promising strategy by many oncologists.