Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Procollagen Type I Propeptide ELISA Kit

Small Sample Reactivity: Mouse N-Term Colorimetric Competition ELISA 3.70 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN7012890
  • Target See all Procollagen Type I Propeptide ELISA Kits
    Procollagen Type I Propeptide
    Binding Specificity
    N-Term
    Reactivity
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    3.70 ng/mL - 300 ng/mL
    Minimum Detection Limit
    370.4 pg/mL
    Application
    ELISA
    Purpose
    The kit is a small sample competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide.
    Sensitivity
    1.44 ng/mL
    Grade
    Small Sample
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Sample Volume
    25 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards,
    2. Add 25μL standard or sample to each well.
      Then add 25μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 50μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 50μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 25μL Stop Solution. Read at 450 nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit is not used up all at once, remove only the strips and reagents for the current experiment and leave the remaining strips and reagents in the desired condition.
    2. Standard - Reconstitute the standard with the Standard Diluent, keep it at room temperature for 10 minutes and shake it gently (do not let it foam). Please prepare tubes with Standard Diluent and make a dilution series. Mix each tube thoroughly before the next transfer. The last tube with Standard Diluent is the blank as 0 mg/mL.
    3. Detection Reagent A and Detection Reagent B - Spin or centrifuge the stock of Detection Reagent A and B briefly before use. Dilute to working concentration (1:100) with Assay Diluent A or B, respectively.
    4. Wash Solution - Dilute 10 mL of Wash Solution Concentrate (30x) with 290 mL of deionized or distilled water to make 300 mL of Wash Solution (1x).
    5. TMB Substrate - Aspirate the required amount of solution with sterile tip and do not return the residual solution back into the vial.

    Note:

    1. Serial dilution directly in the wells is not recommended.
    2. Prepare standard within 15 minutes before assay. Do not dissolve the reagents directly at 37 °C.
    3. Detection Reagent A and B are sticky solutions, so pipette them slowly to reduce volume errors.
    4. Reconstitute Standard or working solutions of Detection Reagent A and B carefully according to instructions, avoiding foaming and mixing gently until crystals are completely dissolved. To minimize inaccuracy caused by pipetting, use small volumes and ensure pipettes are calibrated. It is recommended to aspirate more than 10 µL for one-time pipetting.
    5. The reconstituted Standard, Detection Reagent A and B can only be used once.
    6. When crystals have formed in the Wash Solution concentrate (30x), warm it to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or preparation containers affect the detection result.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 ° C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    Expiry Date
    6 months
  • Target See all Procollagen Type I Propeptide ELISA Kits
    Procollagen Type I Propeptide
    Alternative Name
    Procollagen I Propeptide (Procollagen Type I Propeptide Products)
    Background
    P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide
You are here:
Support