NEFL ELISA Kit

Catalog No. ABIN6999487

Product Details NEFL ELISA Kit

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 4.94 pg/mL - 400 pg/mL

Minimum Detection Limit: 4.94 pg/mL

Application: ELISA

Purpose: The kit is a high sensitivity competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Sample Type: Cerebrospinal Fluid, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide.

Sensitivity: 1.95 pg/mL

Grade: High Sensitivity

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Sample Volume: 50 µL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Reagent Preparation:

Reconstitute the Standard with 1.0mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 400pg/mL. Prepare 5 tubes containing 0.6mL Standard Diluent and produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 400pg/mL, 133.33pg/mL, 44.44pg/mL, 14.81pg/mL, 4.94pg/mL, and the last tubes with Standard Diluent is the blank as 0pg/mL.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 ° C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Expiry Date: 6 months

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament, Light Polypeptide (NEFL Products)

Synonyms: NEFL ELISA Kit, nefl ELISA Kit, zgc:136626 ELISA Kit, AI847934 ELISA Kit, CMT2E ELISA Kit, NF-L ELISA Kit, NF68 ELISA Kit, Nfl ELISA Kit, CMT1F ELISA Kit, NFL ELISA Kit, XNF-L ELISA Kit, neurofilament light ELISA Kit, neurofilament, light polypeptide ELISA Kit, neurofilament, light polypeptide b ELISA Kit, neurofilament, light L homeolog ELISA Kit, NEFL ELISA Kit, neflb ELISA Kit, Nefl ELISA Kit, nefl.L ELISA Kit

Background: CMT1F, CMT2E, NF-L, NF68, NFL, 68 kDa neurofilament protein, Neurofilament triplet L protein