HSPA9 ELISA Kit

Catalog No. ABIN6963529

Product Details HSPA9 ELISA Kit

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This kit recognizes Human HSP-70/HSPA9 in samples. No Significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.

Sensitivity: 0.47 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Reagent Preparation:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 50 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 50 ng/mL working solution to the first tube and mix up to produce a 25 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 12 months

References for HSPA9 ELISA Kit (ABIN6963529)

Li, Liu, Zang, Zhou, Zhang, Sun, Qi, Li, Kong, Jin, Yang, Luo, Lu, Lin, Niu, Liu: "Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner." in: Cancer letters, Vol. 492, pp. 71-83, (2021) (PubMed).

Yuan, Dai, Li, Zheng, Zhao, Dong: "Bexarotene Improve Depression-Like Behaviour in Mice by Protecting Against Neuro-inflammation and Synaptic Damage." in: Neurochemical research, Vol. 45, Issue 7, pp. 1500-1509, (2020) (PubMed).

Target Details for HSPA9

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Alternative Name: Heat Shock 70 kDa Protein 9 (HSPA9 Products)

Synonyms: APG-2 ELISA Kit, HS24/P52 ELISA Kit, HSPH2 ELISA Kit, RY ELISA Kit, hsp70 ELISA Kit, hsp70RY ELISA Kit, CSA ELISA Kit, GRP-75 ELISA Kit, GRP75 ELISA Kit, HSPA9B ELISA Kit, MOT ELISA Kit, MOT2 ELISA Kit, MTHSP75 ELISA Kit, PBP74 ELISA Kit, Hspa9a ELISA Kit, csa ELISA Kit, grp-75 ELISA Kit, grp75 ELISA Kit, hspa9 ELISA Kit, hspa9b ELISA Kit, mortalin ELISA Kit, mot ELISA Kit, mot2 ELISA Kit, pbp74 ELISA Kit, mot-2 ELISA Kit, mthsp75 ELISA Kit, 74kDa ELISA Kit, Csa ELISA Kit, Grp75 ELISA Kit, Hsc74 ELISA Kit, Hsp74 ELISA Kit, Hsp74a ELISA Kit, Mortalin ELISA Kit, Mot-2 ELISA Kit, Mot2 ELISA Kit, Mthsp70 ELISA Kit, Pbp74 ELISA Kit, cb740 ELISA Kit, crs ELISA Kit, wu:fc14d08 ELISA Kit, wu:fc27c10 ELISA Kit, wu:fc38a06 ELISA Kit, heat shock protein family A (Hsp70) member 4 ELISA Kit, heat shock protein family A (Hsp70) member 9 ELISA Kit, heat shock protein family A member 9 ELISA Kit, heat shock protein family A (Hsp70) member 9 S homeolog ELISA Kit, stress-70 protein, mitochondrial ELISA Kit, heat shock protein 9 ELISA Kit, heat shock protein Hsp9 ELISA Kit, HSPA4 ELISA Kit, HSPA9 ELISA Kit, Hspa9 ELISA Kit, hspa9.S ELISA Kit, hspa9 ELISA Kit, LOC577721 ELISA Kit, hsp9 ELISA Kit

Background: HSPA9, CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74