Angiotensin II ELISA Kit

Catalog No. ABIN6953754

Product Details Angiotensin II ELISA Kit

Target: Angiotensin II (Ang II)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 24.69 pg/mL - 2000 pg/mL

Minimum Detection Limit: 24.69 pg/mL

Application: ELISA

Purpose: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII)

Sensitivity: 8.84 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Reagent Preparation:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 2,000pg/mL. Please prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or container for reagent preparation will influence the detection result.

Sample Preparation:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Expiry Date: 6 months

References for Angiotensin II ELISA Kit (ABIN6953754)

Jiang, Zhu, Bu, Li, Shao, Gu, Kong, Luo, Long, Guo, Tian, Zhao, Cen: "Brain Renin-Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity." in: Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, Vol. 15, Issue 2, pp. 500-510, (2019) (PubMed).

Tripathi, Sullivan, Fan, Wang, Sun, Reed, Gladysheva: "Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189315, (2018) (PubMed).

Liu, Zhang, Wei, Yuan, Xue, Hao, Xu, Wang, Chen: "ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in cardiomyocytes." in: International journal of cardiology, Vol. 257, pp. 150-159, (2018) (PubMed).

Wang, Qian, Zhao, Xing, Sun: "β-Aminoisobutyric acid ameliorates the renal fibrosis in mouse obstructed kidneys via inhibition of renal fibroblast activation and fibrosis." in: Journal of pharmacological sciences, Vol. 133, Issue 4, pp. 203-213, (2017) (PubMed).

Guo, Namekata, Kimura, Harada, Harada: "The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis." in: The American journal of pathology, Vol. 187, Issue 12, pp. 2876-2885, (2017) (PubMed).

Lin, Zeng, Gao, Gu, Wang, Zhang: "Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression." in: Frontiers in physiology, Vol. 8, pp. 782, (2017) (PubMed).

Yang, Pan, Zhan, Zhang, Guo, Jin: "Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2968462, (2016) (PubMed).

Zhang, Chen, Zhang, Xiao, Ding, Goltzman, Miao: "Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1?(OH)ase knockout mice." in: Neuroscience letters, Vol. 588, pp. 184-9, (2015) (PubMed).

Camacho Londoño, Tian, Hammer, Schröder, Camacho Londoño, Reil, He, Oberhofer, Mannebach, Mathar, Philipp, Tabellion, Schweda, Dietrich, Kaestner, Laufs, Birnbaumer, Flockerzi, Freichel, Lipp: "A background Ca2+ entry pathway mediated by TRPC1/TRPC4 is critical for development of pathological cardiac remodelling." in: European heart journal, Vol. 36, Issue 33, pp. 2257-66, (2015) (PubMed).

Wang, Sukhova, Liu, Ozaki, Lesner, Libby, Kovanen, Shi: "Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice." in: Journal of vascular surgery, (2014) (PubMed).

Wang, Liu, Ye, Zhang, Tang, Chen, Zhang, Lou: "Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes." in: European journal of clinical investigation, Vol. 39, Issue 7, pp. 561-7, (2009) (PubMed).

Wang, Ye, Peng, Tang, Liu, Chen, Yu, Lou: "Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes." in: Nephrology (Carlton, Vic.), Vol. 14, Issue 2, pp. 213-8, (2009) (PubMed).

Target Details for Angiotensin II

Target: Angiotensin II (Ang II)

Alternative Name: Angiotensin II (AngII) (Ang II Products)

Synonyms: ANHU ELISA Kit, SERPINA8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, AngII ELISA Kit, PAT ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity