CBX5 ELISA Kit

Catalog No. ABIN6951753

Product Details CBX5 ELISA Kit

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CBX5 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: CBX5 (CBX5 Products)

Synonyms: HP1 ELISA Kit, HP1A ELISA Kit, id:ibd2026 ELISA Kit, wu:fc63e02 ELISA Kit, wu:fe47g12 ELISA Kit, wu:fi03h05 ELISA Kit, zgc:158601 ELISA Kit, CBX5 ELISA Kit, cbx5 ELISA Kit, MGC64354 ELISA Kit, 2610029O15Rik ELISA Kit, C75991 ELISA Kit, Hp1a ELISA Kit, Hp1alpha ELISA Kit, chromobox 5 ELISA Kit, chromobox homolog 5 (HP1 alpha homolog, Drosophila) ELISA Kit, chromobox 5 S homeolog ELISA Kit, CBX5 ELISA Kit, cbx5 ELISA Kit, Cbx5 ELISA Kit, cbx5.S ELISA Kit

Gene ID: 23468

UniProt: P45973