YWHAB ELISA Kit

Catalog No. ABIN6951750

Product Details YWHAB ELISA Kit

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human YWHAB ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: YWHAB (YWHAB Products)

Synonyms: GW128 ELISA Kit, HS1 ELISA Kit, KCIP-1 ELISA Kit, YWHAA ELISA Kit, 1300003C17Rik ELISA Kit, wu:fb22g04 ELISA Kit, wu:fr86a12 ELISA Kit, ywhab ELISA Kit, ywhab2 ELISA Kit, zgc:64092 ELISA Kit, 14-3-3beta ELISA Kit, fb80c08 ELISA Kit, fk41f08 ELISA Kit, wu:fb80c08 ELISA Kit, wu:fd11d01 ELISA Kit, wu:fk41f08 ELISA Kit, ywhab1 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a ELISA Kit, YWHAB ELISA Kit, Ywhab ELISA Kit, ywhabb ELISA Kit, ywhab.S ELISA Kit, ywhab.L ELISA Kit, ywhaba ELISA Kit

Gene ID: 7529

UniProt: P31946

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location