WARS ELISA Kit

Catalog No. ABIN6951745

Product Details WARS ELISA Kit

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human WARS ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for WARS

Target: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Alternative Name: WARS (WARS Products)

Synonyms: GAMMA-2 ELISA Kit, IFI53 ELISA Kit, IFP53 ELISA Kit, zgc:73274 ELISA Kit, wars ELISA Kit, MGC53671 ELISA Kit, WARS ELISA Kit, TrpRS ELISA Kit, WRS ELISA Kit, 85D-WRS ELISA Kit, CG9735 ELISA Kit, Dmel\\CG9735 ELISA Kit, WRS-85D ELISA Kit, l(3)03559 ELISA Kit, l(3)03560 ELISA Kit, l(3)3559 ELISA Kit, gamma-2 ELISA Kit, ifi53 ELISA Kit, ifp53 ELISA Kit, GB14934 ELISA Kit, ECK3371 ELISA Kit, JW3347 ELISA Kit, tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase S homeolog ELISA Kit, Tryptophanyl-tRNA synthetase ELISA Kit, tryptophanyl-tRNA synthetase L homeolog ELISA Kit, tryptophan--tRNA ligase, cytoplasmic ELISA Kit, WARS ELISA Kit, wars ELISA Kit, wars.S ELISA Kit, Wars ELISA Kit, TrpRS ELISA Kit, wars.L ELISA Kit, LOC727582 ELISA Kit, trpS ELISA Kit, trpS2 ELISA Kit

Gene ID: 7453

UniProt: P23381