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FAM171B ELISA Kit
FAM171B
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details FAM171B ELISA Kit
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Target
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FAM171B
(Family with Sequence Similarity 171, Member B (FAM171B))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Human FAM171B ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Expiry Date
6 months
Target Details for FAM171B
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Target
See all FAM171B products
FAM171B
(Family with Sequence Similarity 171, Member B (FAM171B))
Alternative Name
FAM171B (FAM171B Products )
Synonyms
KIAA1946 ELISA Kit, D430039N05Rik ELISA Kit, family with sequence similarity 171 member B ELISA Kit, family with sequence similarity 171, member B ELISA Kit, FAM171B ELISA Kit, Fam171b ELISA Kit
Gene ID
165215
UniProt
Q6P995
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