ATG12 ELISA Kit

Catalog No. ABIN6951723

Product Details ATG12 ELISA Kit

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATG12 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 (ATG12 Products)

Synonyms: APG12 ELISA Kit, APG12L ELISA Kit, FBR93 ELISA Kit, HAPG12 ELISA Kit, Apg12l ELISA Kit, AUTOPHAGY 12 ELISA Kit, AUTOPHAGY 12 B ELISA Kit, CG10861 ELISA Kit, Dmel\\CG10861 ELISA Kit, atg12 ELISA Kit, ATG12 ELISA Kit, apg12 ELISA Kit, DDBDRAFT_0185323 ELISA Kit, DDBDRAFT_0191412 ELISA Kit, DDB_0185323 ELISA Kit, DDB_0191412 ELISA Kit, 4931423H11Rik ELISA Kit, A330058M13Rik ELISA Kit, Atg12l ELISA Kit, AO090001000593 ELISA Kit, autophagy related 12 ELISA Kit, Ubiquitin-like superfamily protein ELISA Kit, Atg12p ELISA Kit, autophagy protein ELISA Kit, ubiquitin-like protein ATG12B ELISA Kit, autophagy associated protein Atg12 ELISA Kit, Autophagy-related 12 ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene ELISA Kit, autophagy associated ubiquitin-like modifier Atg12 ELISA Kit, hypothetical protein ELISA Kit, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) ELISA Kit, autophagy protein 12 ELISA Kit, autophagy related 12 S homeolog ELISA Kit, autophagy-related protein 12 ELISA Kit, ATG12 ELISA Kit, Atg12 ELISA Kit, APG12B ELISA Kit, APG12 ELISA Kit, LOC9321066 ELISA Kit, apg12 ELISA Kit, atg12 ELISA Kit, LOC743425 ELISA Kit, CAALFM_C113950CA ELISA Kit, atg12.S ELISA Kit, ANI_1_922094 ELISA Kit, AOR_1_1064164 ELISA Kit

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy