NCKIPSD ELISA Kit

Catalog No. ABIN6951696

Product Details NCKIPSD ELISA Kit

Target: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human NCKIPSD ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for NCKIPSD

Target: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Alternative Name: NCKIPSD (NCKIPSD Products)

Synonyms: AF3P21 ELISA Kit, DIP ELISA Kit, DIP1 ELISA Kit, ORF1 ELISA Kit, SPIN90 ELISA Kit, VIP54 ELISA Kit, WASLBP ELISA Kit, WISH ELISA Kit, Wasbp ELISA Kit, SH3 adapter protein SPIN90 ELISA Kit, NCK interacting protein with SH3 domain ELISA Kit, CpipJ_CPIJ019280 ELISA Kit, NCKIPSD ELISA Kit, Nckipsd ELISA Kit

Gene ID: 51517

UniProt: Q9NZQ3

Pathways: Protein targeting to Nucleus