RNASE1 ELISA Kit

Catalog No. ABIN6951694

Product Details RNASE1 ELISA Kit

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human RNASE1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RNASE1

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Alternative Name: RNASE1 (RNASE1 Products)

Synonyms: RNASE1 ELISA Kit, RNS1 ELISA Kit, ATRNS1 ELISA Kit, RIBONUCLEASE 1 ELISA Kit, T17M13.16 ELISA Kit, T17M13_16 ELISA Kit, ribonuclease 1 ELISA Kit, RIB1 ELISA Kit, AI574248 ELISA Kit, Rib-1 ELISA Kit, Rib1 ELISA Kit, SRN ELISA Kit, ribonuclease A family member 1, pancreatic ELISA Kit, ribonuclease pancreatic-like ELISA Kit, ribonuclease A family member k6 ELISA Kit, Ribonuclease 1 ELISA Kit, ribonuclease 1 ELISA Kit, ribonuclease, RNase A family, 1 (pancreatic) ELISA Kit, ribonuclease pancreatic ELISA Kit, RNASE1 ELISA Kit, LOC475395 ELISA Kit, RNASE6 ELISA Kit, Rnase1 ELISA Kit, RNS1 ELISA Kit, LOC101113761 ELISA Kit

Gene ID: 6035

UniProt: P07998