PHGDH ELISA Kit

Catalog No. ABIN6951683

Product Details PHGDH ELISA Kit

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human PHGDH ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH (PHGDH Products)

Synonyms: fb38f06 ELISA Kit, zgc:65956 ELISA Kit, wu:fb38f06 ELISA Kit, embryo sac development arrest 9 ELISA Kit, F10M10.7 ELISA Kit, ECK2909 ELISA Kit, JW2880 ELISA Kit, 3-PGDH ELISA Kit, 3PGDH ELISA Kit, 4930479N23 ELISA Kit, A10 ELISA Kit, PGAD ELISA Kit, PGD ELISA Kit, PGDH ELISA Kit, SERA ELISA Kit, PDG ELISA Kit, phosphoglycerate dehydrogenase ELISA Kit, D-3-phosphoglycerate dehydrogenase ELISA Kit, 3-phosphoglycerate dehydrogenase ELISA Kit, phgdh ELISA Kit, EDA9 ELISA Kit, serA ELISA Kit, Sthe_2284 ELISA Kit, Dacet_1260 ELISA Kit, Mrub_0173 ELISA Kit, MMAH_RS07650 ELISA Kit, Arnit_0487 ELISA Kit, Ndas_0174 ELISA Kit, Mesil_2386 ELISA Kit, Slip_0010 ELISA Kit, Trad_0302 ELISA Kit, Acear_0027 ELISA Kit, Mahau_0665 ELISA Kit, MZHIL_RS05165 ELISA Kit, Mesop_1331 ELISA Kit, Thein_0855 ELISA Kit, Phgdh ELISA Kit, PHGDH ELISA Kit

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect